| We are located within the Daniel Rutherford building on The University of Edinburgh's King's Buildings campus. Our facilities are equipped for the culture of fungal cells, advanced light microscopy and basic molecular biology. We are permitted to work with containment level two organisms. |
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The main lab is equipped for fungal culture, basic molecular biology, including standard DNA cloning and PCR, biochemistry and luminometry.
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Hera Safe laminar flow for general sterile work including culture handling and propagation, and a fume cupboard for handling hazardous substances and to run and stain DNA and protein gels. |
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Some of our incubators which run at various temperatures ranging from 20°C to 37°C, with programmable light cycles. We use Sanyo MIR-153 and Gallenkamp Cooled incubators. Additionally, we use a shaking incubator for liquid cultures.
The building communal facilities include:
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Nikon Eclipse TE-2000E workstation for brightfield, phase contrast and DIC imaging. Equiped with Photonics Polychrome IV monochromator as epifluorescence light source. Images are captured using either a Nikon DXM 1200F colour camera or a Hamamatsu Orca-ER CCD camera for quantitative imaging.
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Confocal Laser Scanning Microscope: BioRad Radiance 2100 unit attached to Nikon Eclipse TE-2000E. Various laser sources cover almost the full visible light spectrum for excitation. Additionally, a mercury vapour lamp is attached for epifluorescence.
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Two LCD screens allow for convenient image acquisiton and data managment as well as preliminary image processing.
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Advanced image processing and analysis is performed in our imaging suite equipped with:
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The main office provides desk space for postdocs, PhD students and honours students. Additional desk space is also available in the imaging suite. |
